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Most relevant scientific articles
• Gaitan-Penas H., Gradogna A., Laparra-Cuervo L., Solsona C., Fernandez-Duenas V., Barrallo- Gimeno A. et al. Investigation of LRRC8-Mediated Volume-Regulated Anion Currents in Xenopus Oocytes. Biophysical Journal. 2016;111(7):1429-1443.
Hightlights
In 2016, we have been studying the relationship between the GlialCAM/MLC1 proteins and the chloride channels ClC-2 and LRRc8/VRAC. We have observed that there is a direct interaction between GlialCAM/ MLC1 and ClC-2 which depends on the depolarization potential of astrocytes. These results are in revision in Human Molecular Genetics. On the other hand we wanted to study the new chloride channel LRRC8
and its relationship with MLC1. To this end, we setup the characterization of the channel in Xenopus oocytes, which have been published in Biophysical Journal. When analyzing its functional relationship with MLC1, we have seen that they interact in an indirect manner. We hypothesize that MLC1 regulates different transduction signal events that influence on this channel. We have also participated (articles are now being written) in the characterization of mutants found in ClC-2 in patients with the leukodystrophy CC2L, in finding the degradation mechanisms of MLC1 at the plasma membrane, in the generation and characterization of a knockout of the ClC-a channel in Drosophila, in an structural characterization of mutations in GlialCAM and in the generation and characterization of knockout of GlialCAM in the zebrafish. On the other hand, we have characterized novel mutations in the ClC-1 chloride channel found in myotonia (results published in 2016 in Human Mutation) and other mutations (article in progress). We have also generated knockout models in zebrafish of the chloride channels ClC-k and ClC-1, which characterization is in progress.
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research groups 77