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cultures, and a mouse model of nasopharyngeal colonization. Besides, the impact

of risky behaviors like smoking that facilitates bacterial colonization of the lungs

and that contributes to the acute exacerbations in patients with chronic obstruc-
tive pulmonary disease will also be examined. Finally, one of the main aims of

the present project is to develop prophylactic and therapeutic approaches to fight
pneumococcal colonization. This will be performed using CWHs (enzybiotics) like

Cpl-7 (a phage-coded enzyme of great antibacterial potential), and novel drugs

including several choline analogs and ceragenins. As for other objectives of this
project, the efficacy of enzybiotics and novel drugs will be tested in vitro (plank-

tonic as well as biofilm cultures) and in animal models of infection.

Most relevant • doMenecH M, rAMoS-SeVillAno e, gArcíA e, MoScoSo M, YuSte J. Biofilm formation avoids
complement immunity and phagocytosis of Streptococcus pneumoniaeInfection and í
scientific
Immunity. 2013;81:2606-2615.
articles
• deZ-MArtíneZ r, de PAZ H, BuStAMAnte n, gArcíA e, MenéndeZ M, gArcíA P.. Improving the

lethal effect of Cpl-7, a pneumococcal phage lysozyme with broad bactericidal acti- í
vity, by inverting the net charge of its cell wall-binding moduleAntimicrobial Agents ó

and Chemotherapy. 2013;57:5355-5365.
éó
• doMenecH M, gArcíA e, Prieto A, MoScoSo M. Insight into the composition of the inter-
cellular matrix of Streptococcus pneumoniae biofilmsEnvironmental Microbiology.

2013;15:502-516.
ó

Highlights
In addition to that provided by CIBER, our research team is supported by grants í
from Plan Nacional de Investigación:

• Colonizacin neumoccica y estado de portador: bases moleculares, profilaxis y
ó
medidas teraputicas. SAF2012-39444-C02-01 and -02. 2013-2015.

• Tecnologa para cardiologa y neumococo (NanoCardioCoco). IPT-2011-1337-

010000. 2011-2014.

Besides, apart from the scientific publications indicated above, a PhD. thesis was
developed in our laboratory and defended in 2013 that have been partially sup-
ó
ported by CIBERES:

Elisa Ramos Sevillano. Enfermedad neumoccica invasiva: mecanismos molecu-

lares de patogenicidad y proteccin. Universidad Complutense de Madrid. Facultad
de Ciencias Biolgicas. 2013. Sobresaliente cum laude.

http://eprints.ucm.es/22886/.

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