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Most relevant scientific articles
• Gómez-Hernández a., Perdomo l., de las Heras n., Beneit n., esCriBano t., otero Y.F. et al . Antagonistic effect of TNF-alpha and insulin on uncoupling protein 2 (UCP-2) expression and vascular damage . Cardiovascular Diabetology . 2014;13(1) .
• GarCía-Guerra l., Vila-Bedmar r., CarrasCo-rando m., CruCes-sande m., martín m., ruiz-Gómez a. et al . Skeletal muscle myogenesis is regulated by G protein-coupled receptor kinase 2 . Journal of Molecular Cell Biology . 2014;6(4):299-311 .
• Bartolomé a., Guillén C. Role of the mammalian target of rapamycin (mTOR) complexes in pancreatic β-cell mass regulation . Vitamins and Hormones . 2014;95:425-469 .
• Bartolomé a., kimura-koYanaGi m., asaHara s.-i., Guillen C., inoue H., teruYama k. et al . Pancrea- tic b-Cell failure mediated by mTORC1 hyperactivity and autophagic impairment . Diabetes . 2014;63(9):2996-3008 .
Highlights
Institution: Universidad Complutense de Madrid Contact: Facultad de Farmacia . Univ . Complutense de Madrid . Ciudad Universitaria, S/N . 28040 Madrid . Phone: (+34) 91 394 17 77 · E.mail: [email protected] · Website: http://www.ciberdem.org
The reconstitution of iLIRKO with IRA isoform by means of adenoviral transfection, but not with isoform IRB, reverts hepatic insulin resistance, glucose intolerance and plasma hyperinsulinemia . More importantly, pancreatic beta cell hyperplasia is being retracted . In pancreatic beta cells, we have demonstrated that the Nicotanimide induces acetylation of TSC2, mTORC1 signaling pathway and cell proliferation, autophagy being downregulated . Resveratrol deacetylates TSC2, this effect being mediated by Sirt- 1, inhibits mTORC1 and cell proliferation and upregulated autophagy . Thus, acetylation/deacetylation status of TSC2 mediated by Sirt-1 constitutes a novel molecular mechanism that regulates autophagy and apoptosis . Regarding the association between vascular insulin resistance and damage, we have demonstrated the protective role of oleic acid, but not palmitate, on the insulin resistance, activation of the proinflammatory pathway NF-Kb and cell proliferation in aortic SMVCs induced by TNF-alpha . Regarding the role of the energy balance on the adipose organ inflammatory disease, we have generated and developed a new IGFIR brown adipose tissue specific knockout, BATIGFIRKO . KO mice lose 25 % of UCP1 expression at 12 months . However, interscapular brown adipose tissue develops normally as compared with their controls, which might be explained by the overexpression of the insulin and BMP- 7 protein machinery observed in the KO mice . More importantly, brown adipose tissue thermogenic function as estimated by cold acclimation was partially impaired . The expression of UCPs in insulin target tissues shows a differential profile . Thus, whereas the expression of UCP2 was increased in the liver or UCP-3 in the heart, the expression of UCP3 in the skeletal muscle was diminished . In the same way, the insulin signaling in vivo was also differentially affected . Thus, insulin signaling remained unchanged in inguinal and epididymal adipose tissue, and also in the heart and skeletal muscle . However, the insulin signaling in the liver was severely impaired associated to lipid accumulation in the liver in the KO mice . Overall, KO mice showed a manifest insulin resistance versus controls .
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