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Most relevant • serra D, Mera P, MalanDrino Mi, Mir JF, herrero l. Mitochondrial fatty acid oxida-
tion in obesity.Antioxid Redox Signal. 2013 Jul 20;19(3):269-84.
scientific
• r M M, h-h F, G M M, e M, r x, F-l
articles
oMeroDelolMGrenolMrasaDelsteVeeMesarernánDezóPez
Ja et al.. Modulation in Wistar rats of blood corticosterone compartmentation by

sex and a cafeteria diet.PLoS One. 2013;8(2):e57342.

• raMírez s, Martins l, Jacas J, carrasco P, Pozo M, clotet J et al.. Hypothalamic
ceramide levels regulated by CPT1C mediate the orexigenic effect of ghrelin.

Diabetes. 2013 Jul;62(7):2329-37.

• MaKowsKi K, Mera P, PareDes D, herrero l, ariza x, asins G et al.. Differential phar-

macologic properties of the two C75 enantiomers: (+)-C75 is a strong anorectic
drug; (-)-C75 has antitumor activity.Chirality. 2013 May;25(5):281-7.

• s D, F-l Ja, r x, a M. The use of TranswellsTM im-
aBaterernánDezóPezeMesarleMany
proves the rates of differentiation and growth of cultured 3T3L1 cells.Anal Bioa-

nal Chem. 2013 Jun;405(16):5605-10.

Our group has shown that both the CPT1A and CPT1C are involved in hypotha-
Highlights
lamic modulation of intake. After fasting, or a dose of ghrelin, an increase in
hypothalamic levels of ceramide occurs. These are mediated by CPT1C and are

required to increase the expression of neuropeptides orexigenic NPY and AgRP.
Furthermore, we have shown that the C75, synthetic racemic product that acts as

an anticancer agent, also has anorexigenic activity and that activity is due to the

inhibitor of the (+ )-C75 enantiomer on CPT1 activity. This opens new avenues for
studying derivatives of this compound that are able to suppress appetite through

inhibition of hypothalamic CPT1. Finally, we have shown that an increase in fatty

acid oxidation due to an overexpression of CPT1A in organs like the liver prevents
obesity in mice fed fat diet.

We have determined the role of sex and cafeteria diet on the partitioning of plas-

ma corticosterone. Cafeteria diet increased the expression of liver CBG gene,
binding plasma capacity and the proportion of blood cell-bound corticosterone.

The use of a monoclonal antibody ELISA and a polyclonal Western blot for plasma

CBG compared with both specific plasma binding of corticosterone and CBG gene
expression suggested the existence of different forms of CBG, with varying affini-

ties for corticosterone in males and females

We also improved methodology for 3T3L1 cell culture, using Transwells TM. The

methodological improvements presented here allow for more uniform cultured
cell yields and a more flexible environment for control of cell size and administra-

tion of signaling agents.

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